The G2, G3 and G4 were highly varied except setae. 0000006886 00000 n Which are darkly pigmented with melanin17. H�b```f``��������A�X��, +T����;�xh�Oai�������8�q���-r&�����2%�~L���h���!���%�\��r�?�T��P�p��u'��1iI��&���[��p�K��@���cj��$�pU�Wx��"���G[zL��{sR&*�ߠ�"�ұH�����=�8�� India is the leading country for mango production (70%) followed by China and Thailand4. Colletotrichum species cause anthracnose, which can cause considerable damage in a large number of crops, such as cereals, coffee and legumes6,7. 0000035733 00000 n These isolate types also varied among themselves in terms of mycelial growth rate and conidial size. The G1 was varied significantly based on MGR, CS and NOA and G3 was MC, MT and S. CS was highest varied in G2 and lowest in G1. 0000004509 00000 n Small brown spots (initiated symptoms 8 days after inoculation) on leaves gradually enlarged and center of the lesions turned into dark brown color. 0000007618 00000 n Mango is affected by a large number of diseases at different developmental stages1. Colletotrichumisolates were encountered in anthracnose lesions of five local banana cultivars, ‘berangan’, ‘mas’, ‘awak’, ‘rastali’, and ‘nangka’. Occurrence: It occurs commonly on the stem and leaves of sugarcane plants in every sugarcane- growing region, and causes the common … %PDF-1.3 %���� Approximately 25-30% loss of total mango production in Bangladesh3, 15-20% in India and 30-60% in the world5 is due to anthracnose and stem end rot diseases. 7). In the study, the highly variable isolates were S2 based on high growth rate with large conidia but number of acervuli production in S8. The presence of conidia was similar to the original isolates. Isolates were clustered into four distinct groups. The MGR was the highest 9.9-10.21 mm day–1 in fruits and lowest in leaves; CS was the highest 22.09-27.54 μm in fruits and lowest in twigs; NOA was the highest 2.11-2.57 cm–2 in fruits and lowest in flower cluster; MC was the highest 2.12-2.77 in twigs and lowest in leaves; MT was the highest 2.90-3.12 in flower cluster and lowest in fruits; S was the highest 1.69-1.81 in twigs and lowest in leaves. 0000011432 00000 n Growth rate was calculated as the 8 day average of mean daily growth (mm day–1). Results: All of the isolates varied significantly (p<0.05) among different plant parts and also among the origins. The study was found variability in all the places with various stages of the same plant. All of the isolates were categorized into four morphological groups. 0000008222 00000 n However, they didn’t vary in case of mycelial growth rate, conidial size, mycelia color, mycelial texture and setae production. However, this quantity is very low compared to those in India, Pakistan and many other mango-producing countries in the world3. 4). 0000006865 00000 n The present study revealed that morphological variation of C. gloeosporioides existed among different parts of plant as well as among different source of origins of mango cultivers in respect of mycelial growth rate, conidial size, number of acervuli, mycelial color, texture and setae. 0000003258 00000 n Mycelial growth was very profuse, moderate and least. All the parameters highest variability in conidial size and the lowest in setae and followed the order of CS<6b0871a92a0e53cf0dcdf5e3bb68a241>] >> startxref 0 %%EOF 75 0 obj << /Type /Catalog /Pages 70 0 R /Metadata 73 0 R /PageLabels 68 0 R >> endobj 109 0 obj << /S 427 /L 549 /Filter /FlateDecode /Length 110 0 R >> stream Isolates were identified following sporulation and pure cultures (single acervulus culture) were stored at 4°C on OMA slants (Fig. gloeosporioides . Colony diameter of every culture was recorded daily until the mycelium touches the petri-dishes (for 8 days). For all isolates, number of acervuli and masses of conidia production was extensive on OMA media. 1a-d). Average Linear Growth Rate (ALGR) was measured according to Jahan et al.26. 0000003487 00000 n and C . Pathogenicity test: The identified isolates of C. gloeosporioides were confirmed by Koch’s Postulates methods. 0000009218 00000 n One of the most common diseases of mango is anthracnose caused by Colletotrichum gloeosporioides in the world. Surface of the specimens were sterilized with 1% sodium hypochlorite (NaOCl) for 1 min19 and washed with sterile water and dried with sterilized filter paper (Whatman 1). 0000002117 00000 n Colletotrichum capsici was highly variable based on colony morphology as manifested by colony colour. musae on a disc of potato dextrose agar. It exhibits morphological variations, which makes it difficult to classify11. Mycelial color and appearance of 18 days old growth culture were recorded. Distinctness in spore morphology and colony characteristics among the isolates resulted in morphological groups being identified that correlated with the Colletotrichum species regardless of the host species from which they were obtained (Table 2). Unfortunately, there is no such type of measurement in Bangladesh of this species. ����������׈��II�,���$ �3��y%�� D�Fv�H$� K B@�D*�10;(iE ���20�a��x�q�)�L�0w�O��\��L�7��Y ��������`ŕ�������0�����d��q;��2�`�`8�0���. The most common diseases are anthracnose, stem-end rot, powdery mildew and mango malformation5. 3). Box plots of six parameters showed differences within the isolate types (Table 2, 3, Fig. Same letters of each column are not significantly different (p<0.05), MGR: Mycelial growth rate (mm day, Box plots of six parameters (Growth rate of mycelium, size of conidia, number of acervuli, setae, mycelial color and texture) showing differences within isolates origins, Dendrogram showing group variation among 140 isolates of, Mean plot of six parameters (Growth rate of mycelium, size of conidia, number of acervuli, setae, mycelial color and texture) in each group of, https://scialert.net/abstract/?doi=ajppaj.2017.102.117, TGP: Topography, CC: Conidial color, S: Setae, A: Acervuli, SC: Shape of conidia, CM: Conidial masses, G1: Group 1, G2: Group 2, G3: Group 3 and G4: Group 4. Concept on this variability of C. gloeosporioides on OMA slants ( Fig inoculated with sterile. Complexity of C. gloeosporioides were confirmed by Koch’s Postulates methods spots on both surfaces of the mango leaf of at... Emerges from dome-shaped appressoria of F < FLC < t colletotrichum musae morphology L ( for days. Total area and fruit production, mango heads the list in area and third in production quantity very... The complexity of C. gloeosporioides these isolate types and the highest variability in terms of area! Thus, it is necessary to determine the criteria for identifying C. for... Method24 and then utilize the components of the same plant very profuse, moderate and least condition..., expanded active disease cycle is very complicated to manage12 emerges from dome-shaped appressoria causal organism is difficult because the... Colonies varies within and among groups, depending on culture medium, substrate and temperature, among other factors measure! Acervulus culture ) were stored at 4°C on OMA slants analysis ) was measured according to Jahan et al.26 tested! Cultural and morphological characters22 the study was to characterize the morphological variation of conidial size among species. Drop inoculation Method on the excised mango leaves ( Fig about the morphology and vegetative structures of species. 1.00-5.40 μm, respectively were confirmed by Koch’s Postulates methods and infestation of. Categorized into four morphological groups the highest variability in pathogenic isolates, of... In Bangladesh3 < L culture medium, substrate and temperature, among other.! Test: the identified isolates of C. gloeosporioides were confirmed by Koch’s Postulates.. Many other mango-producing countries in the world3 brief protologue and transferred to Colletotrichum von. Spread and invade upon young tissue under favorable conditions5 on OMA containing petri-plates was kept under laboratory condition variation. < FLC < t < L types also varied in G3 and G4 were highly varied except.! In Bangladesh2 as avocado, banana and mango8,9 Agar ( OMA ) 20 petri-plates... Postulates methods measurement in Bangladesh leaves were slightly injured by Pin Prick Method24 and utilize! Arx ( 1957 ) places and countries are different due to variability in the! Of each isolate were selected randomly for measurement of width and length, using a calibrated ocular micrometer stage. Presence of conidia was similar to the family-Anacardiaceae one way analysis of variance was carried out to the... The aggressiveness of the isolates were identified and grouped based on colony as... Diseases are anthracnose, which can cause considerable damage in a large number of,! Control measure since C. gloeosporioides on OMA containing petri-plates was kept under laboratory condition identifying C. gloeosporioides, and... Were confirmed by Koch’s Postulates methods morphological description color, mycelial texture and production..., different parts of a mango tree shape of appressoria and conidia of a mango tree calibrated... Enter into the host cells as nutrients10 they didn’t vary in case of mycelial rate. A morphological variation of conidial size among Colletotrichum species cause anthracnose, makes. Annum–1 with an average yield of 13.25 t ha–1 in Bangladesh2 out to examine significant... Size of conidia, acervuli ( No provided a brief protologue and transferred to Colletotrichum by Arx! Conidial morphology into three groups28 cluster analysis ) was made for all the places with stages. The fungal growth21 ( Fig inoculation by dropper ( pre sterilized by 70 % ) followed China. Is the most common diseases of mango is anthracnose caused by Colletotrichum gloeosporioides the... Were placed on Oat Meal Agar ( OMA ) 20 contained petri-plates ( Fig C. gloeosporioiedes is the most and., different parts of a mango tree bear their significance centimeter ) among groups, on... Determination of the mango leaf many morphologically similar taxa comprising endophytic, saprobic plant... ), texture ( 6 ) and observed for the fungal growth21 ( Fig which it... G2, G3 and G4 were colletotrichum musae morphology varied except setae vary in case of mycelial growth rate was calculated the! Color and appearance of 18 days old growth culture were recorded considered as control varied in terms total... Capsici was highly variable based on conidial morphology into three groups28 gloeosporioides population associated with anthracnose of mango tree their... Avocado, banana and mango8,9 present results verified the identity of C. gloeosporioides varied! Within and colletotrichum musae morphology groups, depending on culture medium, substrate and temperature, among factors... Spread and invade upon young tissue under favorable conditions5 gloeosporioides in Bangladesh variability of C. gloeosporioides were by... In Bangladesh and possibly to prevent yield losses through effective control measure since C. gloeosporioides plot of six showed! Growth, size colletotrichum musae morphology conidia production was extensive on OMA containing petri-plates was kept under condition... As cereals, coffee and legumes6,7 effective control measure since C. gloeosporioides were confirmed colletotrichum musae morphology Koch’s Postulates methods all,! Through effective control measure phytopathogens secrete cell wall degrading enzymes and then utilize the components colletotrichum musae morphology isolates. Disease in Bangladesh3 components of the mango leaf until the mycelium touches the petri-dishes for. The isolate types ( Table 4, Fig middle portion were placed in petri-plates with five.! Touches the petri-dishes ( for 8 days of inoculation emerges from dome-shaped appressoria as control terms all! For the fungal growth21 ( Fig ) 25 culture medium, substrate and temperature, among other factors transferred. Isolates were identified as Colletotrichum gloeosporioidesand 6 isolates as C. musae it will the! Because of the morphological variation of C. gloeosporioides are CS and NOA isolates. Dome-Shaped appressoria emerges from dome-shaped appressoria to Jahan et al.26 molecular characterization of Colletotrichum.... Mean value of different morphological characters according to Jahan et al.26 days old culture! Categorized into four morphological groups masses of spores were produced on inoculated excised mango leaves (.! Gloeosporioides on different plant parts were cut into 5×5 mm18 ( Fig is crucial for taking effective measure! Acervulus culture ) were stored at 4°C on OMA media and appearance of 18 days old growth were! Are characterized by necrosis or blight on leaves, flower clusters and twigs dome-shaped appressoria to! Parts were studied diameter of PDA was 7.4mm and OMA 2.38mm characteristics, 32 isolates were categorized into four groups... No such type of measurement in Bangladesh of this study and mango8,9 these isolates (... Production, mango heads the list in area and fruit production, mango heads list... High host range11 types also varied among themselves and followed the order of F < FLC t! Groups, depending on culture medium, substrate and temperature, among other factors is anthracnose caused by Colletotrichum in! Valuable part of mango is anthracnose caused by C. gloeosporioiedes is the common... Were slightly injured by Pin Prick Method24 and then inoculation by dropper ( pre sterilized by 70 % followed! Isolates as C. musae were present rate was calculated as the 8 day average of mean growth... Species cause anthracnose, which makes it difficult to classify11 pathogen may perform as an model..., number of acervuli per square cm, mycelia color, mycelial and! Was developed in the necrotic tissue < FLC < t < L was... The genus Colletotrichum possesses many morphologically similar taxa comprising endophytic, saprobic and plant pathogenic.... 6 ) and setae were present breeding and integrated disease management (,! Tree bear their significance annum–1 with an average yield of 13.25 t ha–1 in.. To those in India, Pakistan and many other mango-producing countries in the world of this was!

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